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The biochemical composition of the turbot skin was investigated. The moisture level of the skin was found to be 51.4%. Based on dry matter content, there were relatively high protein(82.1%) and lipid(13.1%) concentrations in the turbot skin. Mineral element analysis revealed that the turbot skin had high Ca content(2069.0 mg kg-1), and the concentrations of toxic heavy metals Hg and Pb were less than 0.005 mg kg-1, which indicates that the turbot skin is a safe resource for collagen production. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis showed that acid-soluble collagen(ASC) extracted from the turbot skin was type-I collagen. The imino acid content of the ASC was 241.6 per 1000 amino acids, which suggests a relatively high denaturation temperature. The Fourier transform infrared(FTIR) spectra of ASC reflected a highly stable structure, and the measured denaturation temperature of ASC was 29.5℃, which is higher than those from many temperate fishes. ASC was the most soluble at pH 4.0, and when the pH values were below or above 4.0, the solubility decreased rapidly. The ASC exhibited a relatively high solubility when NaCl concentration was lower than 2%. These results show that turbo skin can be employed as a source for producing collagen with high quality. 相似文献
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居民幸福感研究日益受到重视,以往研究多是从自然环境、社会人文环境和城市化进程等方面探讨大尺度空间环境因素对城市居民幸福感的影响。结合乡村旅游的角度探讨城市居民幸福感的影响研究,目前还较少。运用SEM分析方法,探究处于多维压力之下的城市居民旅游者如何通过前往乡村进行旅游休闲活动提升幸福感,进行模型构建与分析,结果显示:① 压力源对调适策略有显著正向影响,对休闲参与不显著,但不同群体影响差异显著,压力调适对休闲参与有显著正向影响;② 休闲参与对心流体验和幸福感具有显著正向关系,且心流体验对幸福感具有显著正向影响;③ 心流体验对休闲效益具有正向显著影响,且休闲效益对幸福感具有正向显著影响;④ 心流体验在休闲参与对幸福感、休闲效益在心流体验对幸福感均具有显著中介效应;⑤ 旅游者不同背景变项在压力调适、休闲参与及幸福感等分别呈现不同程度的显著差异。 相似文献
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1-phenylethanol(1-PEA)is a flavor extensively used in the production of co smetics,beverages,and food.The release of 1-PEA into coastal environments has aroused great concern.However,its potential effects on marine organisms are still unknown.In order to provide a better understanding of the ecological risks of 1-PEA in marine environments,this study determined the toxic effects of 1-PEA on two marine diatoms(Phaeodactylum tricornutum and Skeletonema costatum).The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h.The contents of chlorophyll a,chlorophyll c,glutathione(GSH),malondialdehyde(MDA),and the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx),were measured at the end of the exposure period.1-PEA was shown to significantly inhibit the growth of diatoms,with 96-h EC_(50) values of 257.14 mg/L and 126.46 mg/L in P.tricornutum and S.costatum,respectively.In P.tricornutum,the levels of SOD,CAT,GPx,GSH,and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC_(50)value.However,in S.costatum,the activities of SOD and CAT,and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC_(50) value.Taken together,these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms. 相似文献
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成熟的倾斜摄影测量及密集匹配技术可获取密集的城市点云,对点云进行三角网构建是真三维建模的关键技术之一。提出了基于区域生长的三角网构建方法,使用双约束条件提高点云的搜索效率,基于点边表数据结构进行网格生长,利用夹角约束和三角形孔洞修补优化网格。实验证明,该方法获取的三角网能够准确地表达楼体、桥状建筑等在内的城市实体,网格优化后的孔洞比例约为1‰并且该方法具有一定的抗噪能力。 相似文献
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YUNJUN YAO SHAOHUA ZHAO HUAWEI WAN YUHU ZHANG BO JIANG KUN JIA MENG LIU JINHUI WU 《Journal of Earth System Science》2016,125(6):1245-1253
Terrestrial latent heat flux (LE) in the Three-River Headwaters region (TRHR) of China plays an essential role in quantifying the amount of water evaporation and carbon sink over the high altitude Tibetan Plateau (TP). Global warming is expected to accelerate terrestrial hydrological cycle and to increase evaporation. However, direct field observations are lacking in this region and the long-term variability in LE remains uncertain. In this study, we have revised a semi-empirical Penman LE algorithm based on ground eddy covariance (EC) observations from an alpine grass site and provided new satellite-based evidence to assess LE change in the TRHR during 1982–2010. Our results show that the average annual terrestrial LE in the TRHR is about 38.8 W/m 2 and there is no statistically significant change in annual LE from 1982 to 2010. We also found that during the same time period, terrestrial LE over the east region of the TRHR significantly decreased, on average, by 0.7 W/m 2 per decade, which was driven primarily by the surface incident solar radiation (Rs) limitation, offsetting the increased LE over the west region of the TRHR caused by the increased precipitation (P) and soil moisture (SM). 相似文献
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无内标-多外标校正激光剥蚀等离子体质谱法测定磁铁矿微量元素组成 总被引:4,自引:2,他引:2
激光剥蚀电感耦合等离子体质谱(LA-ICP-MS)常用于磁铁矿原位微量元素分析,按照校正策略不同,主要分为内标法和无内标法。内标法需要用电子探针(EMPA)预先测定磁铁矿中内标元素Fe的含量,过程较繁琐,且待测元素含量会受到内标元素含量测定的影响。本文采用铁含量较高的玄武质玻璃BCR-2G、BIR-1G、BHVO-2G和GSE-1G作为外标,避免了内标元素含量的测定,建立了无内标-多外标校正LA-ICP-MS测定磁铁矿微量元素组成的分析方法。利用该方法测定了科马提岩玻璃GOR-128g和自然岩浆磁铁矿BC 28的微量元素组成以评估方法的可靠性。结果表明,科马提岩玻璃的测定结果与推荐值及前人内标法的测定值一致,多数元素的相对标准偏差RSD5%;自然岩浆磁铁矿的测定结果与推荐值相比,多数元素的RSD7%,低于前人内标法的RSD(15%)。由此说明无内标-多外标法可以实现富铁硅酸岩或磁铁矿微量元素含量的准确校正,克服了基体效应的影响。因此,无内标-多外标法是一种原位测定磁铁矿微量元素含量的快速、准确方法,具有一定的应用潜力。 相似文献
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